| Фрагментация ДНК сперматозоидов: связь с мужским бесплодием и методы коррекции / И. Д. Громенко [и др.] // Патологическая физиология и экспериментальная терапия. – 2023. – Т. 67, № 3. – С. 142–148.
(Sperm DNA fragmentation: association with infertility and methods of correction) |
In 2021, the assessment of sperm DNA fragmentation level was included in the World Health Organization guidelines as a diagnostic method for male infertility. The inability to conceive naturally, abnormal embryo development, increased miscarriage rates, decreased live birth rates, and decreased effectiveness of in vitro fertilization, intracytoplasmic sperm injection, and intrauterine insemination are all associated with high levels of sperm DNA fragmentation. The major causes that compromise the integrity of the genetic material of gametes include errors in chromatin condensation, incomplete apoptosis, and oxidative stress. Among the methods to overcome infertility associated with high levels of DNA fragmentation are: elimination of modifiable risk factors (smoking, varicocele, obesity), antioxidant therapy, short withdrawal period, use of donor oocytes in ART programs, as well as cell selection methods in ICSI programs and use of testicular spermatozoa obtained by testicular biopsy. |
| Геворкян, Н. М. Повышение эффективности терапии стволовыми клетками при содействии суммарных РНК лимфоцитов здоровых доноров / Н. М. Геворкян // Патологическая физиология и экспериментальная терапия. – 2023. – Т. 67, № 3. – С. 124–136.
(Enhancing the effect of stem cell therapy by healthy donor total lymphocyte RNA support) |
It is obvious that stem cells in the body require particularly strict regulation of their activity by integral systems. Previous analysis of the cellular pathogenesis of various diseases and its relationship with disorders of the T-lymphocyte regulation showed that, in pathological conditions, there are always functional disorders in a series of morphogenetic reparative T-lymphocytes that control the homeostasis. Previously, in vivo and in vitro experiments have provided qualitative and quantitative evidence that regulatory properties of total RNA from the spleen, thymus or peripheral blood lymphocytes are consistent with the properties of the lym-phocytes themselves, and these properties are manifested in recovery processes of organs and tissues. It has also been shown that preparations of total RNA from allogeneic or xenogeneic lymphocytes of a healthy donor contribute to the “reprogramming” of the recipient’s lymphocytes, their normalization, and, thus, the restoration of impaired functions in experimental animals. Accord-ingly, we suggest to precede the administration of stem cells by the administration of total RNA from lymphoid cells of healthy donors to enhance the recovery of disordered functions of a specific organ system. |
| Шамитова, Е. Н. Гепсидин и его роль в человеческом организме (литературный обзор) / Е. Н. Шамитова, В. Э. Петрова, Л. Г. Хамитова // Вестник новых медицинских технологий. – 2023. – Т. 30, № 4. – С. 61–66.
(Hepsidin and its role in the human body) |
One of the hormones involved in iron metabolism is hepcidin. The discovery of its biological properties has clarified the relationship between iron homeostasis, immune response and anemia in chronic diseases, and determining the level plays an important role in their treatment. The review highlights the current modern scientific data on the nature of the iron-regulating hormoneThe research purpose was to study the literature data on the structure, structure and role of hepcidin in the human body. Materials and methods. A systematic review of domestic and foreign literature. Results and its discussion. Hepcidin was discovered in January 1998. It has iron-regulating, antibacterial effects. An increase in the concentration of hepcidin indicates an excess of iron in the blood, and a decrease, on the contrary, indicates iron deficiency. Both conditions – pathological Determination of the level of this hormone also affects the diagnosis of various chronic diseases. Conclusion.Further study of the molecular mechanisms of consumption, use and regulation of hepcidin metabolism will help to understand the pathogenetic aspects of the course of various diseases that violate hepcidin homeostasis. |